DNA Extraction and Purification
Omega Biotek Inc Ultra-Pure Plasmid DNA 96 Kit, 4 x 96 preps
Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format.
IBI SCIENTIFIC 3P MINI I-BLUE PLSMID KT 100 PREP
DNA Purification Kit, I-Blue Mini Plasmid Kit (100-Prep), IBI Scientific, Sample Size: 1-7ml bacterial culture, Operation Time: 20min, Expectant Yield: up to 50ug of pure plasmid DNA, Elution Volume: 30-100ul, Format: Plasmid Spin Column.
Norgen Biotek Corp Stool DNA Isolation Kit, 50 Prep
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Boca Scientific Inc GeneFix 2ml DNA/RNA Saliva Collectors, Pack of 50
GeneFix is an easy-to-use saliva collection device that stabilizes DNA and viral RNA in tubes pre-filled with a non-toxic stabilization buffer. GFX-02 is developed for 2ml of saliva. The collector has a simple screw-on funnel for saliva delivery directly into the buffer. The saliva collection tube clearly indicates the volume of saliva required. Once in the buffer, DNA is stabilized for 5 years and viral RNA is stabilized for 5 days at room temperature, with high yields maintained. With leakproof collection tubes, GeneFix saliva collectors are fully specified for sample transport and storage.
Research Products International Corp DNA Clean & Concentrator-5 (50 Preps) w/ Zymo-Spin IC Columns (Capped)
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Zymo Research Corporation ZymoBIOMICS™ Lysis Solution, 150 ml
Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Omega Biotek Inc E.Z.N.A. FastFilter Plasmid Maxi Kit | 25 preps
The E.Z.N.A. FastFilter Plasmid Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation.
Research Products International Corp Quick-DNA/RNA MiniPrep Plus Kit, 50 Preps
The Quick-DNA/RNA MiniPrep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality genomic DNA and total RNA that is ready for any downstream application including reverse transcription, microarray, sequencing, etc.
Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
New England Biolabs, Inc. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® – 24 reactions
The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities. The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.
- Get more of what you need, with the highest library yields
- Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
- Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
- Improve yields and quality for target enrichment applications
Zymo Research Corporation Quick-DNA™ FFPE MiniPrep (50 preps)
For easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Bulldog Bio Inc FastGene Scriptase II ReadyMix (100 rxns), Random Hex-primers
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
Zymo Research Corporation Genomic DNA Wash 2, 12 ml
Genomic DNA Wash 2 (12 ml). Sold separately or as part of a nucleic acid purification kit from Zymo Research.
Qiagen, Inc. QIAEX II GEL EXTRACT KIT (150)
Kit, Gel Extraction; Qiagen; QIAEX II (150); For batch purification of DNA fragments from agarose gels and solutions; Contains: 3 x 0.5mL QIAEX II Suspension, Buffers; For 150 extractions